Multiple-locus variable-number tandem repeat analysis for strain typing of Clostridium perfringens
Youhanna S Sawires1, J Glenn Songer
1Department of Veterinary Science and Microbiology, University of Arizona, Room 201, 1117 East Lowelll Street, Tucson, AZ 85721, USA.
Anaerobe
|May 17, 2006
Summary
A new Multiple-Locus Variable-Number Tandem Repeat Analysis (MLVA) scheme efficiently types Clostridium perfringens strains. This rapid, cost-effective method aids in understanding C. perfringens epidemiology and evolution.
Area of Science:
- Microbiology
- Genetics
- Epidemiology
Background:
- Clostridium perfringens is a widespread bacterium causing various diseases in humans and animals.
- Effective strain typing is crucial for understanding C. perfringens epidemiology, virulence evolution, and ecological impact.
Purpose of the Study:
- To develop and evaluate a Multiple-Locus Variable-Number Tandem Repeat Analysis (MLVA) scheme for Clostridium perfringens strain typing.
- To assess the typability, reproducibility, and discriminatory power of the developed MLVA scheme.
Main Methods:
- Development of an MLVA scheme targeting five variable tandem repeat (VNTR) loci in C. perfringens.
- Screening of 112 C. perfringens isolates using the developed MLVA scheme.
- Evaluation of typability, reproducibility, and discriminatory power.
Main Results:
- All 112 C. perfringens isolates were successfully assigned an MLVA genotype.
- The MLVA scheme demonstrated excellent reproducibility.
- A high numerical index of discrimination (0.995) was achieved for the five VNTR loci.
Conclusions:
- The developed MLVA scheme is an efficient, rapid, and cost-effective tool for Clostridium perfringens strain typing.
- This PCR-based method is suitable for epidemiological, ecological, and evolutionary studies of C. perfringens.
