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Updated: Aug 8, 2026

Self-Assembly of Microtubule Tactoids
Published on: June 23, 2022
Microtubule-bundling activity of APC is stimulated by interaction with PSD-95
Nobutake Takamori1, Atsushi Shimomura, Takao Senda
1Department of Anatomy I, School of Medicine, Fujita Health University, Toyoake, Aichi 470-1192, Japan.
Abstract:
Adenomatous polyposis coli (APC) tumor suppressor protein binds to microtubules, leading to microtubule bundling and stabilization. The protein also interacts with postsynaptic density (PSD)-95, a major scaffolding protein in neurons. Here, we analyzed the effects of PSD-95 on the microtubule-bundling activity of APC. The coexpression of APC and PSD-95 in COS-7 cells enhanced microtubule-bundle formation compared with the expression of APC alone. A mutant APC variant that does not associate with PSD-95 did not enhance microtubule bundling, despite coexpression with PSD-95. Immunoelectron microscopy showed that the APC-PSD-95 complex sometimes colocalized on microtubules in processes of cultured neurons. These results suggest that the microtubule-bundling activity of APC is regulated by its interaction with PSD-95, which might modulate microtubule architecture and dynamics in neurons.
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