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Universal genotyping in tuberculosis control program, New York City, 2001-2003.
Carla M Clark1, Cynthia R Driver, Sonal S Munsiff
1Tuberculosis Control Program, New York City Department of Health and Mental Hygiene, 225 Broadway, New York, NY 10007, USA. cclark@health.NYC.gov
Emerging Infectious Diseases
|May 18, 2006
Summary
New York City
Area of Science:
- Microbiology
- Epidemiology
- Public Health
Background:
- Tuberculosis (TB) control in New York City.
- Implementation of molecular genotyping for TB surveillance.
Purpose of the Study:
- Evaluate the utility of IS6110 restriction fragment length polymorphism (RFLP) and spoligotyping in TB control.
- Identify transmission links, false-positive cultures, and new TB cases.
Main Methods:
- Genotyping of Mycobacterium tuberculosis isolates using IS6110 RFLP and spoligotyping.
- Analysis of genotyping data to identify patient clusters and transmission events.
Main Results:
- Genotyping successfully identified 205 clusters, revealing previously unknown transmission links and sites.
- Fifty-nine false-positive cultures were detected, reducing unnecessary treatment.
- Four additional TB cases were identified through cluster investigations.
Conclusions:
- Genotyping significantly enhanced TB surveillance and control efforts in NYC.
- Molecular typing is crucial for identifying transmission dynamics and improving patient outcomes.