Related Experiment Video

Updated: Aug 8, 2026

Optogenetic Inhibition of Rho1-Mediated Actomyosin Contractility Coupled with Measurement of Epithelial Tension in Drosophila Embryos
12:35

Optogenetic Inhibition of Rho1-Mediated Actomyosin Contractility Coupled with Measurement of Epithelial Tension in Drosophila Embryos

Published on: April 14, 2023

Cyclin D1 regulates cellular migration through the inhibition of thrombospondin 1 and ROCK signaling

Zhiping Li1, Chenguang Wang, Xuanmao Jiao

  • 1Thomas Jefferson University, Department of Cancer Biology, Kimmel Cancer Center, Bluemle Building, Rm 1050, 233 South 10th St, Philadelphia, PA 19107, USA.

Molecular and Cellular Biology
|May 18, 2006
PubMed

Insights

Cyclin D1, overexpressed in tumors, promotes cell motility by inhibiting Rho-activated kinase II (ROCKII) signaling and repressing metastasis suppressor thrombospondin 1 (TSP-1). Loss of cyclin D1 increases adhesion and decreases motility.

Area of Science:

  • Oncology
  • Cell Biology
  • Molecular Biology

Background:

  • Cyclin D1 is frequently overexpressed in human tumors and linked to metastasis.
  • Activation of Rho GTPases induces cyclin D1 expression.
  • The precise role of cyclin D1 in regulating cell adhesion and motility remains incompletely understood.

Purpose of the Study:

  • To investigate the role of cyclin D1 in regulating cellular adhesion, motility, and associated molecular pathways.
  • To identify genes regulated by cyclin D1 that influence cell migration.
  • To elucidate the mechanism by which cyclin D1 affects cell metastasis.

Main Methods:

  • Utilized cyclin D1-deficient mouse embryo fibroblasts (MEFs) and wild-type MEFs for comparative analysis.
  • Employed retroviral transduction to reintroduce cyclin D1 into deficient cells.
  • Performed mutational analysis of cyclin D1, genomewide expression arrays, and Rho GTPase activity assays.

Main Results:

  • Cyclin D1 deficiency led to increased cell adhesion and decreased motility, which were reversed by cyclin D1 reintroduction.
  • Cyclin D1 regulates genes including Rho-activated kinase II (ROCKII) and thrombospondin 1 (TSP-1).
  • Cyclin D1-deficient cells exhibited elevated Rho GTP, ROCKII activity, and downstream signaling, alongside repressed ROCKII and TSP-1 expression.

Conclusions:

  • Cyclin D1 promotes cellular motility by inhibiting ROCK signaling pathways.
  • Cyclin D1 acts as a repressor of the metastasis suppressor TSP-1.
  • Targeting cyclin D1 or its downstream effectors may offer therapeutic strategies for inhibiting cancer metastasis.

More Related Videos

Impedance-based Real-time Measurement of Cancer Cell Migration and Invasion09:23

Impedance-based Real-time Measurement of Cancer Cell Migration and Invasion

Published on: April 2, 2020

Quantitative Analysis of Random Migration of Cells Using Time-lapse Video Microscopy07:27

Quantitative Analysis of Random Migration of Cells Using Time-lapse Video Microscopy

Published on: May 13, 2012

Related Concept Videos

Inhibition of Cdk Activity02:34

Inhibition of Cdk Activity

The orderly progression of the cell cycle depends on the activation of Cdk protein by binding to its cyclin partner. However, the cell cycle must be restricted when undergoing abnormal changes. Most cancers correlate to the deregulated cell cycle, and since Cdks are a central component of the cell cycle, Cdk inhibitors are extensively studied to develop anticancer agents. For instance, cyclin D associates with several Cdks, such as Cdk 4/6, to form an active complex. The cyclin D-Cdk4/6 complex...
Inhibition of CDK Activity02:34

Inhibition of CDK Activity

The orderly progression of the cell cycle depends on the activation of Cdk protein by binding to its cyclin partner. However, the cell cycle must be restricted when undergoing abnormal changes. Most cancers correlate to the deregulated cell cycle, and since Cdks are a central component of the cell cycle, Cdk inhibitors are extensively studied to develop anticancer agents. For instance, cyclin D associates with several Cdks, such as Cdk 4/6, to form an active complex. The cyclin D-Cdk4/6 complex...
Cytoskeletal Coordination in Cell Migration01:32

Cytoskeletal Coordination in Cell Migration

A migrating cell changes its shape during the cyclic events of attachment and detachment from the substratum and repositions the cell organelles correspondingly. These complex events are orchestrated by the dynamic cytoskeletal network comprising actin filaments, intermediate filaments, and microtubules. Cytoskeletal crosstalk — the direct and indirect communication between the different components — is crucial for this coordination. Direct communication involves various linker proteins that...
Cell Migration01:09

Cell Migration

Cell migration, the process by which cells move from one location to another, is essential for the proper development and viability of organisms throughout their life. When cells are not able to migrate properly to their ordained locations, various disorders may occur. For example, disruption in cell migration causes chronic inflammatory diseases such as arthritis.
Cell Migration01:19

Cell Migration

Cell migration is a process by which the cells move from one location to another, playing an essential role in embryological development, repair and regeneration, immune response, and metastasis. Cells migrate in response to chemical or mechanical signals generated by specific organs or tissues. The overall mechanism includes three steps - polarization, protrusion, and release. Polarization involves the formation of a distinct cell front and rear, which determines the direction of movement.
Positive Regulator Molecules02:39

Positive Regulator Molecules

Mitotic cell division results in daughter cells that exactly resemble the parent cell. However, errors in the DNA replication or distribution of genetic material may lead to genetic mutations that may be passed down to every new cell formed from the resulting abnormal cell. Propagation of such mutant cells is restricted through checkpoint mechanisms present at different stages of the cell cycle. These checkpoints involve regulator molecules that either promote or demote cell cycle events.