Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Virus-receptor interaction in poliovirus entry and pathogenesis.

V R Racaniello1

  • 1Department of Microbiology, Columbia University College of Physicians and Surgeons, New York, New York.

Harvey Lectures
|January 1, 1991
PubMed
Summary

Understanding poliovirus infection requires studying virus-receptor interactions beyond structure. Genetic analysis of poliovirus and PVR mutants, combined with structural data, will clarify early infection events and receptor function.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Kinetic analysis of the effect of poliovirus receptor on viral uncoating: the receptor as a catalyst.

Journal of virology·2001
Same author

It is too early to stop polio vaccination.

Bulletin of the World Health Organization·2000
Same author

Two distinct binding affinities of poliovirus for its cellular receptor.

The Journal of biological chemistry·2000
Same author

Defects in nuclear and cytoskeletal morphology and mitochondrial localization in spermatozoa of mice lacking nectin-2, a component of cell-cell adherens junctions.

Molecular and cellular biology·2000
Same author

An antiviral compound that blocks structural transitions of poliovirus prevents receptor binding at low temperatures.

Journal of virology·2000
Same author

Three-dimensional structure of poliovirus receptor bound to poliovirus.

Proceedings of the National Academy of Sciences of the United States of America·2000

Area of Science:

  • Virology
  • Cell Biology
  • Structural Biology

Background:

  • Poliovirus infection mechanisms remain incompletely understood, particularly the early steps of receptor interaction and genome release.
  • While the three-dimensional structure of poliovirus is known, it doesn't fully explain the dynamic processes of infection.
  • Viral receptors, like Poliovirus Receptor (PVR), have essential cellular functions beyond viral entry.

Purpose of the Study:

  • To elucidate the molecular mechanisms of poliovirus entry into host cells.
  • To understand the cellular functions of the Poliovirus Receptor (PVR) and its potential ligands.
  • To investigate the role of Membrane Protein H (MPH) in poliovirus infection and host cell processes.

Main Methods:

  • Genetic and biochemical analysis of poliovirus and PVR mutants.
  • Three-dimensional structural determination of PVR and potentially PVR-poliovirus complexes.
  • Analysis of mice with targeted disruption of the MPH gene.

Main Results:

  • Genetic and structural studies are crucial for understanding virus-receptor binding, alteration, and uncoating.
  • PVR, an Ig superfamily member, likely plays roles in cell adhesion and communication.
  • Investigating PVR ligands and MPH function can reveal insights into normal cellular processes and virus-host interactions.

Conclusions:

  • Combining structural and genetic data is essential for a comprehensive understanding of poliovirus infection.
  • Studying viral receptors like PVR bridges virology and cell biology, potentially uncovering new cellular functions.
  • Understanding the PVR-poliovirus interaction and MPH function may shed light on cellular events triggered by viral infections.

Related Experiment Videos