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[Determination of acetylharpagide in Ajuga decumbens by HPLC]
Dang-Kun Yue1, Guang-Zhi Ding, Wei-Hao Wang
1Institue of Chinese Materia Medica, China Academy of Chinese Medicial Sciences, Bejing 100700, China.
Objective:
To establish a method of the quantitative determination of acetylharpagide in Ajuga decumbens.
Method:
The chromatographic conditions were as follows: a Phenomenex Luna C18 column was used, the mobile phase was composed of acetontrile-water (15:85), the flow rate was 1.0 mL x min(-1) and the UV absorbance detection was set at 197 nm.
Result:
Linearity of acetylharpagide was in the range of 0.6-3.6 microg (r = 0.9993), and the average recovery and RSD were 99.13% and 2.48%, respectively.
Conclusion:
The contents of acetylharpagide ranged 0.40%-6.39% in A. decumbens. The method was simple, accurate and sensitive.