[Proliferative activity of human endothelial cell line EA.hy926 and its modulation]

Angiogenesis is a complicated process, which is regulated by numerous cytokines and growth factors. Besides, the interaction of endothelial cells with extracellular matrix components, with other cell types and with each other is essential for the formation on new blood vessels. The initiation, continuation and completion of angiogenesis depend on the balance of pro- and antiangiogenic factors in the endothelium microenvironment. Factors that influence endothelial cell proliferation are necessary for vascular development and their normal functioning. The influence of new pharmaceutical agents on angiogenesis is commonly evaluated by results of in vivo assays, i.e. chick chorioallantoic membrane and rabbit cornea assays. However, reported results are not always objective. So, the aim of our study was to elaborate methods of estimation of endothelial cell proliferation as one of important stages of angiogenesis.