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VIM-1 metallo-beta-lactamase in Acinetobacter baumannii.

Athanassios Tsakris1, Alexandros Ikonomidis, Spyros Pournaras

  • 1Department of Microbiology, Medical School, University of Athens, Athens, Greece. atsakris@med.uoa.gr

Emerging Infectious Diseases
|May 19, 2006
PubMed
Summary

Five metallo-beta-lactamase (MBL)-positive Acinetobacter baumannii isolates carrying blaVIM-1 were identified in Greek hospitals. This finding is concerning due to Acinetobacter spp.

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Area of Science:

  • Microbiology
  • Infectious Diseases
  • Molecular Biology

Background:

  • Acinetobacter baumannii is a significant nosocomial pathogen.
  • Increasing antimicrobial resistance in Acinetobacter spp. is a global health concern.
  • Metallo-beta-lactamases (MBLs) confer resistance to carbapenems, a last-resort antibiotic class.

Purpose of the Study:

  • To report the emergence of metallo-beta-lactamase (MBL)-producing Acinetobacter baumannii in Greece.
  • To characterize the genetic elements associated with MBL production in these isolates.

Main Methods:

  • Bacterial isolates were identified and characterized using standard microbiological techniques.
  • Molecular methods, including PCR and sequencing, were used to detect and identify beta-lactamase genes.

Related Experiment Videos

  • Integron analysis was performed to understand gene cassette organization.

    • Main Results:

    • Five unrelated Acinetobacter baumannii isolates positive for metallo-beta-lactamase (MBL) were detected in two Greek hospitals between 2004 and 2005.
    • All isolates carried the blaVIM-1 gene within a class 1 integron.
    • Additional carbapenemase genes, blaOXA-51-like and blaOXA-58-like, were also identified in these isolates.

    Conclusions:

    • The presence of VIM-1 MBL-producing Acinetobacter baumannii is a significant public health concern in Greece.
    • The co-occurrence of multiple carbapenemase genes highlights the complex resistance mechanisms in these pathogens.
    • Continuous surveillance is crucial to monitor and control the spread of multidrug-resistant Acinetobacter baumannii.