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[Construction of two hepatocellular carcinoma cell models for the expression of HBV X gene with different selection
Xing-e He1, Jian-hua Lei, Xu Yang
1Liver Disease Research Center, Second Xiangya Hospital, Central South University, Changsha 410011, China.
Objective:
To construct 2 hepatocellular carcinoma (HCC) cell models for the expression of HBV X gene with different selection characteristics.
Methods:
HepG2 HCC cells were infected with eukaryotic expression vectors with HBV X gene, pCEP4-X, and pcDNA3. 1 (+)-X. Single cell clone was selected by hygromycin and neomycin. After propagating culture for certain periods, the HBV X gene expression was identified by PCR, RT-PCR, and Western blot.
Results:
Single HCC cell clone with HBV X gene transferred resistant to hygromycin and neomycin was selectively cultured, and the cells could be propagated for certain periods. PCR, RT-PCR, and Western blot identified the expression of HBV X gene.
Conclusion:
Two HCC cell models for the expression of HBV X gene with different selection characteristics have been successfully constructed.
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