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Updated: Aug 8, 2026

FISH for Pre-implantation Genetic Diagnosis
Published on: February 23, 2011
[Confirmation of the extra small chromosome in abnormality karyotype by PCR and FISH]
Zhi-gang Xue1, Yu-mei Li, Zhong-yuan Yao
1National Key Laboratory of Medical Genetics, Central South University, Changsha 410078, China.
Objective:
To investigate the source of the extra small chromosome in a patient with karyotype 45,X[115]/46,X + mar[45]/46,XY[29].
Methods:
The SRYgene was detected by PCR, and the chromosome Y probe that labeled with biotin was detected by fluorescence in situ hybridization.
Results:
SRY gene is detected positive and the mar chromosome showed positive signal with FISH in human chromosome Y probe pool.
Conclusion:
The extra small chromosome is part of the chromosome Y.
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