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Updated: Aug 8, 2026

A Protocol for Immunohistochemistry and RNA In-situ Distribution within Early Drosophila Embryo
Published on: May 6, 2022
Endoplasmic reticulum generates calcium signalling microdomains around the nucleus and spindle in syncytial
H Parry1, A McDougall, M Whitaker
1Institute for Cell and Molecular Biosciences, Medical School, University of Newcastle upon Tyne, UK.
Abstract:
Cell cycle calcium signals are generated by inositol trisphosphate-mediated release of calcium from internal stores [Ciapa, Pesando, Wilding and Whitaker (1994) Nature (London) 368, 875-878; Groigno and Whitaker (1998) Cell 92, 193-204]. The major internal calcium store is the ER (endoplasmic reticulum): the spatial organization of the ER during mitosis is important in defining a microdomain around the nucleus and mitotic spindle in early Drosophila embryos [Parry, McDougall and Whitaker (2005) J. Cell Biol. 171, 47-59]. Nuclear divisions in syncytial Drosophila embryos are accompanied by both cortical and nuclear localized calcium transients. Mitosis is prevented by the InsP(3) antagonists Xestospongin C and heparin. Nuclear-localized transients and cortical transients rely on extraembryonic calcium, suggesting that ER calcium levels are maintained by calcium influx.
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