Related Experiment Video
Updated: Aug 8, 2026

Preparation of Developing and Adult Drosophila Brains and Retinae for Live Imaging
Published on: March 15, 2010
Drosophila homologue of Eps15 is essential for synaptic vesicle recycling
Amitabha Majumdar1, Shobha Ramagiri, Richa Rikhy
1Department of Biological Sciences, Tata Institute of Fundamental Research, Colaba Mumbai 400005, India. amitavamajumdar@gmail.com
Abstract:
The mammalian protein Eps15 is phosphorylated by EGF receptor tyrosine kinase and has been shown to interact with several components of the endocytic machinery. We have identified a hypomorphic Eps15 mutant in Drosophila which shows reversible paralysis and an altered physiology at restrictive temperatures. In addition, the temperature-sensitive paralytic defect of shibire mutant is enhanced by this mutant. Eps15 is enriched in the larval neuromuscular junction in endocytic 'hot spots' in a pattern similar to Dynamin. Eps15 mutants show a decrease in the alpha-Adaptin levels at the larval neuromuscular junction synapse. Genetic and biochemical studies of interactions with components of the endocytic machinery suggest that Eps15 has an important role in synaptic vesicle recycling and regulates recruitment of alpha-Adaptin.
Related Concept Videos
Overview of Secretory Vesicles
Various proteins regulate the aggregation of molecules inside the secretory vesicles. Chromogranins...
Recycling Endosomes and Transcytosis
The recycling endosome is not a single organelle but an extensively tubulated network of recycling pathways. It functions in storing molecules or transporting them across...
Vesicular Tubular Clusters
With the help of motor proteins such...

