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Updated: Aug 8, 2026

An Ecdysone Receptor-based Singular Gene Switch for Deliberate Expression of Transgene with Robustness, Reversibility, and Negligible Leakiness
Published on: May 7, 2018
Improving the speed of the genetic toggle switch without sacrificing its dynamic stability
1Department of Molecular and Cellular Biology, Harvard University, Cambridge, MA 02138, USA. jrchen@fas.harvard.edu
Abstract:
Determinants of the switching speed of the genetic toggle switch remain unknown. Analysis shows that the decay rate of proteins predominantly sets the speed limit, but its modification introduces a trade-off between increased speed and decreased bistability. Incorporating protein-modifying enzymes into the switch gives extra degrees of freedom to address this trade-off. The condition for bistability when such enzymes are incorporated is derived. Under this condition, speed increases with the maximal rate of gene expression.
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