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Updated: Aug 7, 2026

Deficient Pms2, ERCC1, Ku86, CcOI in Field Defects During Progression to Colon Cancer
28:15

Deficient Pms2, ERCC1, Ku86, CcOI in Field Defects During Progression to Colon Cancer

Published on: July 29, 2010

Pleiotropic-resistant phenotype is a multifactorial phenomenon in human colon carcinoma cell lines

G Toffoli1, A Viel, L Tumiotto

  • 1Division of Experimental Oncology 1, Centro di Riferimento Oncologico, Aviano, Italy.

British Journal of Cancer
|January 1, 1991
PubMed

Insights

Multidrug resistance (MDR) in colon cancer cells involves multiple biochemical mechanisms. These mechanisms confer cross-resistance to certain chemotherapy drugs like doxorubicin but not others.

Area of Science:

  • Oncology
  • Molecular Biology
  • Biochemistry

Background:

  • The multidrug-resistant (MDR) phenotype is a significant challenge in cancer chemotherapy.
  • Understanding the biochemical underpinnings of MDR is crucial for developing effective treatment strategies.

Purpose of the Study:

  • To investigate the biochemical basis of the MDR phenotype in human colon carcinoma cell lines.
  • To analyze the cross-resistance patterns and molecular alterations in drug-selected sublines.

Main Methods:

  • Development of drug-resistant sublines through single-step doxorubicin selection of LoVo, DLD1, and SW948 human colon carcinoma cell lines.
  • Assessment of cross-resistance to doxorubicin (DOX), actinomycin-D (ACT-D), vincristine (VCR), and cis-diamminedichloroplatinum (CDDP).
  • Quantification of mdr1 mRNA expression and gp170 glycoprotein levels.

Main Results:

  • Selected sublines exhibited cross-resistance to DOX, ACT-D, and VCR, but not CDDP.
  • Increased expression of mdr1 mRNA and gp170 glycoprotein was observed in chemoresistant sublines.
  • Comparative analyses revealed varying degrees of resistance mechanisms across different cell lines.

Conclusions:

  • The MDR phenotype in these colon cancer cell lines is a multifactorial phenomenon.
  • Cooperating biochemical mechanisms, varying by cell line, contribute to cellular chemoresistance.
  • Further research into these mechanisms could inform novel therapeutic approaches for MDR cancers.

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