Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Application of a column selection system and DryLab software for high-performance liquid chromatography method

Ryan M Krisko1, Kieran McLaughlin, Michael J Koenigbauer

  • 1Department of Chemistry, University of Kansas, Mallott Hall, Lawrence, KS 66045, USA.

Journal of Chromatography. A
|May 23, 2006
PubMed
Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Differential hepatic activation of mouse and human peroxisome proliferator-activated receptor-α by perfluorohexane sulfonate.

Toxicological sciences : an official journal of the Society of Toxicology·2025
Same author

Occurrence of COVID-19 and serum <i>per</i>- and polyfluoroalkyl substances: A case-control study among workers with a wide range of exposures.

Global epidemiology·2024
Same author

An investigation of 3M Cordova, IL production worker's per- and polyfluoroalkyl substances biomonitoring results and mortality experience.

International journal of hygiene and environmental health·2024
Same author

Antibody response to COVID-19 vaccines among workers with a wide range of exposure to per- and polyfluoroalkyl substances.

Environment international·2022
Same author

Determination of in plasma samples by dual-electrode amperometric detection and liquid chromatography.

Analytical methods : advancing methods and applications·2020
Same author

Evaluation of the effectiveness of a tailored mobile application in increasing the duration of wear of thermoplastic retainers: a randomized controlled trial.

European journal of orthodontics·2019

This study presents a novel strategy for developing chromatographic methods for drug candidates. It uses simple mobile phases and optimizes selectivity via stationary phase and pH variations for efficient separation.

Area of Science:

  • Analytical Chemistry
  • Pharmaceutical Analysis
  • Chromatography

Background:

  • Developing robust chromatographic methods is crucial for drug candidate analysis.
  • Optimizing selectivity and using mass spectrometry (MS)-compatible mobile phases are key challenges.

Purpose of the Study:

  • To describe a systematic strategy for developing high-performance liquid chromatography (HPLC) methods for drug candidates.
  • To achieve efficient separation of diverse analytes using MS-compatible conditions.

Main Methods:

  • Utilized a strategy involving simple MS-compatible mobile phases.
  • Optimized chromatographic selectivity by varying stationary phases and mobile phase pH.
  • Employed an automated column selection system and DryLab software.

Related Experiment Videos

  • Tested the method on bupivacaine and its metabolites, acidic/basic/neutral compounds, and atenolol/nitrendipine degradation products.

    • Main Results:

    • Successfully developed chromatographic methods for complex mixtures.
    • Demonstrated the effectiveness of stationary phase and pH optimization for selectivity.
    • Validated the approach for various drug candidates and related substances.

    Conclusions:

    • The described strategy provides an efficient and systematic approach to developing HPLC methods for drug candidates.
    • The combination of automated systems, varied columns, and software enables rapid optimization.
    • This method facilitates the separation of diverse compounds, including metabolites and degradation products, crucial for pharmaceutical development.