Brain and cognitive impairments from burn injury in rats

Severe burn injuries affect not only the release of stress hormones but also the metabolism of nitric oxide (NO), a substance playing a large role in cognition. We investigated the effect of third-degree burns both on central NO-levels and on short-term memory in rats. Burns were administered under halothane-anesthesia by dipping 20% of the skin area in hot water. In a first experiment, NO-changes were estimated over hours by differential normal pulse voltammetry (DPNV) with a sensor implanted chronically in the frontal cortex. In a second experiment, cognitive abilities were tested over days by comparing the spontaneous time used to explore objects that the animals had, either never- or already-encountered before. Cerebral NO appeared steadily depleted for at least 12h after the injury, not after control anesthesia. During nine days following the burn, discrimination performance was lower compared to controls. Putting together the results of the two experiments, especially on the day of burn, NO changes are likely to account for the behavioral effect. A choice of neuro-pharmacological agents involved in NO-metabolism, together with a choice of proper anesthetics, should now be tested as means to alleviate cognitive impairments following third-degree burns.