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Preparation of Highly Porous Coordination Polymer Coatings on Macroporous Polymer Monoliths for Enhanced Enrichment of Phosphopeptides
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Preparation of Highly Porous Coordination Polymer Coatings on Macroporous Polymer Monoliths for Enhanced Enrichment of Phosphopeptides

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Novel adsorptive polyamine coating for enhanced capillary electrophoresis of basic proteins and peptides

Angel Puerta1, Jakob Axén, Lennart Söderberg

  • 1Department of Physical and Analytical Chemistry, Analytical Chemistry, Biomedical Centre, Uppsala University, PO Box 599, SE-75124 Uppsala, Sweden.

Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
|May 23, 2006
PubMed

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Electrophoresis is a powerful analytical separation technique that relies on the differential migration of charged species when subjected to an electric field. The core strength of electrophoresis lies in its ability to separate high-molecular-weight species in complex mixtures. It has found widespread use in biochemistry, molecular biology, and analytical chemistry, allowing the separation of compounds like amino acids, nucleotides, carbohydrates, and proteins with excellent resolution.
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Two-dimensional gel electrophoresis is a high-resolution protein separation method first introduced by O' Farrell and Klose in 1975. This method involves protein separation by two dimensions, mass and charge, making it more accurate than one-dimensional gel electrophoresis.
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