Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Histologic comparison of autologous fat processing methods.

John G Rose1, Mark J Lucarelli, Bradley N Lemke

  • 1Oculoplastics Service, Department of Ophthalmology and Visual Sciences, University of Wisconsin-Madison, USA.

Ophthalmic Plastic and Reconstructive Surgery
|May 23, 2006
PubMed
Summary

Sedimentation processing for autologous fat transfer yields more viable adipocytes than washing or centrifuging. This method enhances fat graft survival by preserving more intact fat cells.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Intersurgeon Variability in Proptosis Reduction After Orbital Decompression for Thyroid Eye Disease: A Multicenter Analysis.

Ophthalmic plastic and reconstructive surgery·2025
Same author

A Case of Malignant Transformation of an Orbital Epidermoid Cyst to Cystic Squamous Cell Carcinoma.

Ophthalmic plastic and reconstructive surgery·2024
Same author

Periorbital Necrotizing Sweet's Syndrome: A Case Report.

Ophthalmic plastic and reconstructive surgery·2023
Same author

Orbital Artifacts on MRI.

Ophthalmic plastic and reconstructive surgery·2023
Same author

Inferior altitudinal visual loss and mask-wearing practices: A case series.

Indian journal of ophthalmology·2023
Same author

Outcomes of Patients With Thyroid Eye Disease Partially Treated With Teprotumumab.

Ophthalmic plastic and reconstructive surgery·2022

Area of Science:

  • Plastic Surgery
  • Regenerative Medicine
  • Adipose Tissue Engineering

Background:

  • Autologous fat transfer is a common procedure for soft tissue reconstruction.
  • Adipocyte viability after processing is crucial for successful fat graft outcomes.
  • Current fat processing methods include washing, centrifuging, and sedimentation.

Purpose of the Study:

  • To quantitatively compare adipocyte viability across different fat processing techniques.
  • To determine the optimal method for preserving adipocytes during autologous fat transfer.

Main Methods:

  • An experimental study involving 24 fat samples from 22 patients undergoing autologous fat transfer.
  • Histologic analysis using periodic acid-Schiff staining to assess adipocyte integrity.

Related Experiment Videos

  • Quantitative assessment of intact adipocytes, nucleated adipocytes, and adipocyte cross-sectional area.

    • Main Results:

    • Sedimentation processing resulted in significantly higher counts of intact and nucleated adipocytes per high-powered field.
    • Adipocyte cross-sectional area was also significantly greater in samples processed by sedimentation.
    • Washing and centrifuging methods showed lower adipocyte viability compared to sedimentation.

    Conclusions:

    • Sedimentation is a superior method for processing adipose tissue in autologous fat transfer, preserving a higher proportion of viable adipocytes.
    • This finding suggests that sedimentation may lead to improved fat graft survival rates.
    • The study highlights the importance of processing technique in maximizing the efficacy of autologous fat grafting.