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Updated: Aug 8, 2026
11:32
Identification of Transcription Factor Regulators using Medium-Throughput Screening of Arrayed Libraries and a Dual-Luciferase-Based Reporter
Published on: March 27, 2020
p63 regulates an adhesion programme and cell survival in epithelial cells
Danielle K Carroll1, Jason S Carroll, Chee-Onn Leong
1Department of Cell Biology, Harvard Medical School, 240 Longwood Ave, Boston, MA 02115, USA.
Nature Cell Biology
|May 23, 2006
Summary
The transcription factor p63 is crucial for epithelial development, regulating cell adhesion and survival. Loss of p63 causes cell detachment, while its overexpression promotes adhesion and resistance to anoikis.
Area of Science:
- Molecular Biology
- Cell Biology
- Developmental Biology
Background:
- The transcription factor p63 plays a critical role in epithelial development.
- However, the specific transcriptional programs regulated by p63 are not well understood.
Purpose of the Study:
- To investigate the role of p63 in regulating cellular adhesion and survival.
- To characterize the transcriptional changes and cellular effects associated with p63 modulation.
Main Methods:
- Modulation of p63 expression (knockdown and overexpression) in mammary epithelial cells and keratinocytes.
- Analysis of transcriptional changes using gene expression profiling.
- Assessment of cellular adhesion, detachment, and anoikis.
- Investigation of signaling pathways, including beta4 integrin.
Main Results:
- p63 knockdown led to downregulation of cell adhesion genes, cell detachment, and anoikis.
- Overexpression of TAp63gamma or deltaNp63alpha isoforms upregulated cell adhesion molecules and conferred resistance to anoikis.
- Apoptosis resulting from p63 loss was rescued by signaling downstream of beta4 integrin.
Conclusions:
- p63 is a key regulator of cellular adhesion and survival in basal cells of the mammary gland and other stratified epithelial tissues.
- p63 influences epithelial integrity through the regulation of cell adhesion molecules and anoikis resistance.
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