The antigen recognized by MOMA-I is sialoadhesin
Cornelia Oetke1, Georg Kraal, Paul R Crocker
1The Wellcome Trust Biocentre, Division of Cell Biology and Immunology, School of Life Sciences, University of Dundee, UK. c.oetke@dundee.ac.uk
Immunology Letters
|May 24, 2006
Summary
The monoclonal antibody MOMA-1 targets sialoadhesin (Sn), a key macrophage marker. This study confirms MOMA-1 recognizes Sn, clarifying its use in identifying marginal metallophilic macrophages.
Area of Science:
- Immunology
- Cell Biology
- Macrophage Biology
Background:
- Monoclonal antibody MOMA-1 is a common marker for splenic marginal metallophilic macrophages.
- The specific antigen targeted by MOMA-1 has remained uncharacterized.
- MOMA-1 expression patterns resemble those of sialoadhesin (Sn, CD169, Siglec-1).
Purpose of the Study:
- To characterize the antigen recognized by the MOMA-1 antibody.
- To determine if MOMA-1 recognizes sialoadhesin (Sn).
Main Methods:
- Flow cytometry analysis of Sn-transfected cells.
- Immunohistochemical staining of lymphoid tissues from Sn-deficient mice.
Main Results:
- MOMA-1 specifically binds to cells expressing sialoadhesin (Sn).
- MOMA-1 staining is absent in lymphoid tissues of Sn-deficient mice.
- These findings confirm that Sn is the antigen recognized by MOMA-1.
Conclusions:
- The MOMA-1 antibody recognizes sialoadhesin (Sn).
- This characterization validates MOMA-1 as a reliable marker for Sn-expressing macrophages.
- Provides clarity for researchers using MOMA-1 in immunology and cell biology studies.
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