Synthesis and structure analysis of novel disaccharides containing D-psicose produced by endo-1,4-beta-D-xylanase
Hisaka Oshima1, Isao Kimura, Ken Izumori
1Food Research Institute of Kagawa Prefectural Industrial Technology Center, 587-1 Goutou-cho, Takamatsu-shi, Kagawa 761-8031, Japan.
Abstract:
Xylosylpsicoses (XPs) were synthesized by transglycosylation (TG) with arabinoxylan (AX) and D-psicose (D-psi) using endo-1,4-beta-D-xylanase (EC 3.2.1.8) from Aspergillus sojae. The structures of XPs were analyzed by HPLC to determine sugar composition and molecular mass, by methylation analysis using GC-MS to determine linkage, and by 1H- and 13C-NMR spectrometries to obtain the anomeric configuration of glycosidic linkage. By chemical analysis, it was found that the structures of XPs are Xylp beta1-4Psip, Xylp beta1-5Psip, Xylp beta1-1Psif and Xylp beta1-6Psif.
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