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Updated: Jul 29, 2026

Generation of Recombinant Human IgG Monoclonal Antibodies from Immortalized Sorted B Cells
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Generation of Recombinant Human IgG Monoclonal Antibodies from Immortalized Sorted B Cells

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Generation of monoclonal antibodies using simplified single-cell reverse transcription-polymerase chain reaction and

Muhamad Ali1, Kiyotaka Hitomi, Hideo Nakano

  • 1Laboratory of Molecular Biotechnology, Graduate School of Bioagricultural Sciences, Nagoya University, Furo-cho, Chikusa-ku, Nagoya, 464-8601, Japan.

Journal of Bioscience and Bioengineering
|May 24, 2006
PubMed

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Hybridoma Technology01:31

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Hybridoma technology is used for the large-scale production of monoclonal antibodies. Monoclonal antibodies bind to only a single antigenic determinant or epitope. Such antibodies are used in research, diagnostics, and disease therapy. The hybridoma technology established in 1975 by Georges Köhler and Cesar Milstein was awarded the Nobel Prize in Medicine in 1984 for revolutionizing research and therapy.
Hybridoma Selection
Commonly used fusion techniques — electroporation, polyethylene glycol...