Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Epigenetic Regulation

Epigenetic Regulation

Epigenetic mechanisms play an essential role in healthy development. Conversely, precisely regulated epigenetic mechanisms are disrupted in diseases like cancer.

DNA Microarrays

DNA Microarrays

Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...

Epigenetic Regulation

Epigenetic Regulation

Epigenetic changes alter the physical structure of the DNA without changing the genetic sequence and often regulate whether genes are turned on or off. This regulation ensures that each cell produces only proteins necessary for its function. For example, proteins that promote bone growth are not produced in muscle cells. Epigenetic mechanisms play an essential role in healthy development. Conversely, precisely regulated epigenetic mechanisms are disrupted in diseases like cancer.
X-chromosome...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Social determinants of Health and Google Trends: a worldwide infodemiological report.

European review for medical and pharmacological sciences·2024

Same author

The effect of a bundle intervention for ambulatory otorhinolaryngology procedures on same-day case cancellation rate and associated costs.

Anaesthesia·2024

Same author

What is the Prevalence of Low Health Literacy in European Union Member States? A Systematic Review and Meta-analysis.

Journal of general internal medicine·2021

Same author

Direct measurement of vertical forces shows correlation between mechanical activity and proteolytic ability of invadopodia.

Science advances·2020

Same author

Timing of HPV16-E6 antibody seroconversion before OPSCC: findings from the HPVC3 consortium.

Annals of oncology : official journal of the European Society for Medical Oncology·2019

Same author

Histochemistry of the lung of the Australian snake-necked tortoise chelodina longicollis.

Journal of morphology·2018

Search research articles

Related Experiment Video

Updated: Jul 11, 2026

Targeted DNA Methylation Analysis by Next-generation Sequencing

Targeted DNA Methylation Analysis by Next-generation Sequencing

Published on: February 24, 2015

Classification of DNA methylation patterns in tumor cell genomes using a CpG island microarray.

L R Adrien¹, N F Schlecht, N Kawachi

¹Department of Pathology, Albert Einstein College of Medicine, Bronx, NY 10461, USA.

Cytogenetic and Genome Research

|May 24, 2006

Summary

This study profiles DNA methylation in head and neck squamous cell carcinoma (HNSCC) using a novel microarray technique. Findings aim to identify epigenetic markers for HNSCC diagnosis and prognosis.

More Related Videos

Methyl-binding DNA capture Sequencing for Patient Tissues

Methyl-binding DNA capture Sequencing for Patient Tissues

Published on: October 31, 2016

Genome-Wide Analysis of DNA Methylation in Gastrointestinal Cancer

Genome-Wide Analysis of DNA Methylation in Gastrointestinal Cancer

Published on: September 18, 2020

Related Experiment Videos

Last Updated: Jul 11, 2026

Targeted DNA Methylation Analysis by Next-generation Sequencing

Targeted DNA Methylation Analysis by Next-generation Sequencing

Published on: February 24, 2015

Methyl-binding DNA capture Sequencing for Patient Tissues

Methyl-binding DNA capture Sequencing for Patient Tissues

Published on: October 31, 2016

Genome-Wide Analysis of DNA Methylation in Gastrointestinal Cancer

Genome-Wide Analysis of DNA Methylation in Gastrointestinal Cancer

Published on: September 18, 2020

Area of Science:

Oncology
Epigenetics
Genomics

Background:

Head and neck squamous cell carcinoma (HNSCC) is a significant global health concern.
Epigenetic alterations, particularly CpG island hypermethylation, play a crucial role in HNSCC development.
Accurate diagnostic and prognostic markers are needed for effective HNSCC management.

Purpose of the Study:

To epigenetically profile CpG island hypermethylation in HNSCC tissue specimens.
To develop and validate a novel microarray-based assay for DNA methylation analysis.
To identify potential epigenetic markers for HNSCC diagnosis and prognosis.

Main Methods:

Utilized a methylation-specific restriction enzyme (MSRE) analysis on a microarray of 12,288 CpG island clones.
Compared methylation-sensitive (HpaII) and insensitive (MspI) restriction enzyme digestion of single DNA samples.
Assessed assay reproducibility using intraclass correlation (ICC) and hierarchical clustering on 37 HNSCC samples.

Main Results:

The MSRE microarray assay demonstrated good reproducibility, with ICC ranging from 0.68 to 0.85 without data filtering.
Repeated assays achieved over 87% concordance after fluorescence intensity filtering.
Hierarchical clustering revealed distinct DNA methylation profiles among HNSCC samples.

Conclusions:

The developed MSRE microarray technique is a reproducible method for profiling CpG island hypermethylation in HNSCC.
Supervised learning techniques are being applied to associate epigenetic signatures with clinical parameters.
This approach holds promise for identifying novel epigenetic markers for HNSCC diagnosis and prognosis.