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Iron uptake and release by macrophages is sensitive to propranolol
Andrei M Komarov1, Jonathon M Hall, Joanna J Chmielinska
1Division of Experimental Medicine, Departments of Biochemistry & Molecular Biology, The George Washington University Medical Center, 2300 Eye St., NW, Ross Hall, Rm. 441A, Washington, DC 20037, USA. phyamk@gwumc.edu
Abstract:
In this study we have tested the effects of d-propranolol (D-Pro) on the iron uptake, iron release and oxidative response of iron-loaded cells in a cellular model of iron-overload using isolated rat peritoneal macrophages incubated with iron-dextran (Fe-D). Pretreatment of macrophages with D-Pro (5-200 microM) prior to Fe-D exposure decreased the cellular iron content and partially prevented iron release from latex-activated macrophages. Release of reactive oxygen species from activated cells was detected by dichlorodihydrofluorescein (DCDHF, 5 microM) oxidation. We found that loading cells with Fe-D increased their response to latex, which was prevented by the lysosomotropic antioxidant agent D-Pro (10 microM).
