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Updated: Aug 8, 2026
10:41
Ion Exchange Chromatography (IEX) Coupled to Multi-angle Light Scattering (MALS) for Protein Separation and Characterization
Published on: April 5, 2019
Serum iohexol analysis by micellar electrokinetic capillary chromatography
Zak K Shihabi1, Mark E Hinsdale
1Department of Pathology, Wake Forest University School of Medicine, Winston-Salem, NC 27157, USA. zshihabi@wfubmc.edu
Electrophoresis
|May 24, 2006
Summary
This study presents a fast, 4-minute method to measure iohexol in serum using capillary electrophoresis. This technique aids in accurately assessing glomerular filtration rate, crucial for kidney function evaluation.
Area of Science:
- Analytical Chemistry
- Clinical Diagnostics
Background:
- Glomerular filtration rate (GFR) is a key indicator of kidney function.
- Accurate GFR measurement is essential for diagnosing and managing kidney diseases.
- Iohexol is a commonly used contrast agent for GFR assessment.
Purpose of the Study:
- To develop a simple and rapid method for measuring iohexol in serum.
- To enable accurate assessment of glomerular filtration rate (GFR).
Main Methods:
- Direct serum injection onto capillary electrophoresis-MEKC (Microemulsion Capillary Electrophoresis).
- Utilized a high pH buffer and increased SDS concentration.
- Implemented frequent standardization and eliminated sample pretreatment.
Main Results:
- Achieved a rapid measurement time of approximately 4 minutes.
- Demonstrated linearity between 8 and 260 mg/L.
- Reported a relative standard deviation (RSD) of peak height of 2.9% for improved precision.
Conclusions:
- The developed MEKC method is a simple, rapid, and precise technique for serum iohexol measurement.
- This method facilitates accurate GFR assessment.
- Optimized parameters enhance daily precision and simplify the workflow.
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