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Evaluation of the Impact of a New Cooling Cell Processor System on Islet Cell Isolation Facility
Published on: August 11, 2023
Comparison of cooling systems during islet purification
Sue Swift1, Tatsuya Kin, Mohammadreza Mirbolooki
1Clinical Islet Transplant Program, University of Alberta and Capital Health Authority, Edmonton, Alberta, Canada.
Cell Transplantation
|May 25, 2006
Summary
Efficient cooling is crucial for human islet isolation using the COBE 2991 cell separator. The original internal cooling system effectively maintained lower temperatures compared to an air-cooled system, which is vital for preserving islet viability.
Area of Science:
- Cell biology
- Medical devices
- Transplantation science
Background:
- Human islet isolation is critical for type 1 diabetes research and transplantation.
- The COBE 2991 cell separator is used for islet purification via density gradient.
- Effective temperature control during islet isolation is essential but current cooling systems are costly and limited.
Purpose of the Study:
- To investigate and compare the efficiency of two cooling methods for the COBE 2991 cell separator.
- To assess the impact of cooling systems on medium temperature during islet purification.
Main Methods:
- Comparison of an original internal cooling system (polyethylene glycol circulation) with an air-cooled system for the COBE 2991.
- Measurement of cooling time, temperature stability, and medium temperature increase during centrifugation.
Main Results:
- The original system required 20 minutes to stabilize temperature within 4-7°C.
- The air system cooled rapidly but lacked stability.
- Both systems increased medium temperature, but the air system resulted in significantly higher post-centrifugation temperatures (13.3°C vs. 8.7°C).
Conclusions:
- The original internal cooling system is more effective at mitigating heat generated by the COBE 2991 machine.
- Further research is needed to determine the clinical significance of the observed temperature differences during islet purification.
