Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Three-dimensional analysis of single particles by electron microscopy: sample preparation and data acquisition.

Teresa Ruiz1, Michael Radermacher

  • 1Department of Molecular Physiology and Biophysics, University of Vermont, Burlington, USA.

Methods in Molecular Biology (Clifton, N.J.)
|May 25, 2006
PubMed
Summary

This guide provides step-by-step instructions for novice researchers on determining three-dimensional structures using single-particle electron microscopy. It covers sample preparation, data acquisition, and practical tips for high-resolution imaging.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The Trimeric Autotransporter Adhesin EmaA and Infective Endocarditis.

Pathogens (Basel, Switzerland)·2024
Same author

CMR reveals myocardial damage from cardiotoxic oncologic therapies in breast cancer patients.

The international journal of cardiovascular imaging·2023
Same author

Switching to an advanced hybrid closed-loop system in real-world practice improves hypoglycemia awareness and metabolic control in adults with type 1 diabetes, particularly in those with impaired perception of hypoglycemia symptoms.

Diabetes research and clinical practice·2023
Same author

Serotype-Specific Sugars Impact Structure but Not Functions of the Trimeric Autotransporter Adhesin EmaA of Aggregatibacter actinomycetemcomitans.

Journal of bacteriology·2022
Same author

The serotype a-EmaA adhesin of <i>Aggregatibacter actinomycetemcomitans</i> does not require O-PS synthesis for collagen binding activity.

Microbiology (Reading, England)·2022
Same author

Spontaneous Closure of Perimembranous Ventricular Septal Defects: A Janus-Faced Condition.

CASE (Philadelphia, Pa.)·2020

Area of Science:

  • Structural Biology
  • Materials Science
  • Microscopy

Background:

  • Single-particle electron microscopy is a rapidly advancing technique in biological and material sciences.
  • Novice researchers often face challenges in initiating new projects within this field.

Purpose of the Study:

  • To provide a comprehensive guideline for researchers starting projects using single-particle techniques.
  • To offer easy-to-understand, step-by-step instructions for 3D structure determination.

Main Methods:

  • Detailed protocols for support film preparation and various staining techniques.
  • Guidance on assessing sample quality and project viability.
  • In-depth description of vitreous ice preparation for high-resolution data.

Related Experiment Videos

Main Results:

  • The chapter outlines essential techniques for sample preparation and data acquisition.
  • It includes practical tips for optimizing image recording on electron microscopes.
  • The content is designed for both beginners and experienced researchers.

Conclusions:

  • This resource serves as a valuable reference for initiating and executing single-particle electron microscopy projects.
  • It empowers researchers to successfully determine 3D structures with practical insights and techniques.