Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Three-dimensional reconstruction of single particles in electron microscopy image processing.

Michael Radermacher1, Teresa Ruiz

  • 1Department of Molecular Physiology and Biophysics, University of Vermont, Burlington, USA.

Methods in Molecular Biology (Clifton, N.J.)
|May 25, 2006
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The Trimeric Autotransporter Adhesin EmaA and Infective Endocarditis.

Pathogens (Basel, Switzerland)·2024
Same author

CMR reveals myocardial damage from cardiotoxic oncologic therapies in breast cancer patients.

The international journal of cardiovascular imaging·2023
Same author

Switching to an advanced hybrid closed-loop system in real-world practice improves hypoglycemia awareness and metabolic control in adults with type 1 diabetes, particularly in those with impaired perception of hypoglycemia symptoms.

Diabetes research and clinical practice·2023
Same author

Serotype-Specific Sugars Impact Structure but Not Functions of the Trimeric Autotransporter Adhesin EmaA of Aggregatibacter actinomycetemcomitans.

Journal of bacteriology·2022
Same author

The serotype a-EmaA adhesin of <i>Aggregatibacter actinomycetemcomitans</i> does not require O-PS synthesis for collagen binding activity.

Microbiology (Reading, England)·2022
Same author

Spontaneous Closure of Perimembranous Ventricular Septal Defects: A Janus-Faced Condition.

CASE (Philadelphia, Pa.)·2020

This guide explains three-dimensional reconstruction techniques for unknown macromolecules using electron microscopy. It details image processing steps, including alignment and classification, for accurate structural analysis.

Area of Science:

  • Structural biology
  • Biophysics
  • Microscopy

Background:

  • Three-dimensional electron microscopy (3D EM) has advanced significantly, enabling detailed analysis of macromolecular assemblies.
  • Numerous image processing techniques have been developed and widely adopted.
  • Understanding these techniques is crucial for effective application.

Purpose of the Study:

  • To provide a general, step-by-step guide for the 3D reconstruction of unknown macromolecules.
  • To explain the principles and limitations of various image processing techniques.
  • To offer a framework applicable across different image processing systems.

Main Methods:

  • Detailed explanation of two- and three-dimensional alignment techniques.
  • Description of classification procedures for image data.

Related Experiment Videos

  • Guidance on the application of 3D reconstruction algorithms.

    • Main Results:

    • A comprehensive overview of essential image processing steps for 3D EM.
    • Explanation of the limitations associated with different techniques.
    • A generalized approach to macromolecular reconstruction.

    Conclusions:

    • Mastery of 3D EM processing steps is essential for accurate macromolecular structure determination.
    • This guide provides a foundational understanding for researchers utilizing 3D electron microscopy.
    • The described methods facilitate the reconstruction of unknown macromolecular structures.