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[RAPD analysis of Scutellaria baicalensis from different germplasms]
Ai-Juan Shao1, Xin Li, Lu-Qi Huang
1Institute of Chinese Materia Medica, China Academy of Chinese Medicial Sciences, Beijing 100700, China.
Objective:
To provide reference for breeding Scutellaria baicalensis from different germplasms,and to explore their genetic diversities and different strains.
Method:
The RAPD method was applied to the study on S. baicalensis from different germplasms by use of 14 random primers about 10 bp, and SAPD-analysis of 34 S. baicalensis from different germplasms was carried out by using the method of withingroups-linkage in SPSS 10.0.
Results:
14 among 115 primers were selected to amplify about 165 segments of DNA. Among them, 132 segments of DNA, 80.0% of the total, can represent genetic of diversities of S. baicalensis. According to RAPD analysis, 34 germplasms of S. baicalensis were classified into A, B, C and D categories.
Conclusion:
S. baicalensis from different germplasms shows abundant genetic diversities. Germplasms from Shandong like Mengyin 3, Menyin 2 and Pingyi have close distance (0.315) in genetic background, which can be chosen for breeding of cultivated. Though genetic characters are similar in the morphology, the geological distribution of S. baicalensis and its morphology have not certain correlation. The complex genetic background of S. baicalensis indicate that the work of the selective breeding and management for breeding of S. baicalensis have to be strengthened.
