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[Effect of Herba Epimedii flavone on the osteoblasts metabolism in vitro]
Yi-Heng Liu1, Hai-Ying Zhang, Hong-Min Zang
1Department of Orthopedics, Second Hospital of Xi'an Jiaotong University, Xi'an 710004, China. liuyiheng-xjtu@sohu.com
Objective:
To explore the effect of Herba Epimedii flavone (HEF) on the osteoblast metabolism in vitro.
Method:
Osteoblast were obtained from new born rat calvaria by digestive enzymes. MTF, PNPP and RT-PCR were used to observe the proliferation, activity of ALP and mRNA expression of OPG and RANKL of cultured osteoblasts in vitro.
Result:
It was found that HEF had the effect on stimulating cell proliferation, activity of ALP and the mRNA expression of OPG of cultured osteoblasts (P < 0.01, P < 0.05).
Conclusion:
HEF can promote the proliferation, the differentiation and the expression of OPG mRNA of the osteoblasts cultured in vitro.
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