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Related Experiment Videos

Decrease in muscle glucose transporter number in chronic physical inactivity in rats.

T Fushiki1, T Kano, K Inoue

  • 1Department of Food Science and Technology, Faculty of Agriculture, Kyoto University, Japan.

The American Journal of Physiology
|March 1, 1991
PubMed
Summary

Physical inactivity in rats reduced whole body insulin sensitivity and muscle glucose uptake. This decrease in glucose transporter number in muscles contributes to impaired insulin action, highlighting the impact of reduced physical activity.

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Area of Science:

  • Physiology
  • Metabolism
  • Endocrinology

Background:

  • Physical inactivity leads to muscle disuse, impacting metabolic health.
  • Understanding insulin action and glucose transport is crucial for metabolic research.

Purpose of the Study:

  • To investigate the effects of a novel physical inactivity model on whole body insulin action.
  • To examine changes in muscle glucose uptake and glucose transporter numbers in response to inactivity.

Main Methods:

  • Rats were subjected to 14 days of physical inactivity, allowing voluntary contractile activity.
  • Whole body insulin sensitivity was assessed using the euglycemic clamp technique.
  • Muscle glucose transporter levels were quantified using the cytochalasin B binding assay.

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Main Results:

  • Physical inactivity minimally affected body weight gain but significantly reduced gastrocnemius muscle growth.
  • Insulin-stimulated glucose disposal was impaired at higher insulin concentrations (14 mU.kg-1.min-1).
  • Gastrocnemius muscle glucose uptake and glucose transporter numbers were significantly decreased in inactive rats.

Conclusions:

  • A 14-day period of physical inactivity reduces whole body insulin sensitivity in rats.
  • Decreased glucose transporter numbers in muscles contribute to the observed reduction in glucose uptake.
  • This study provides insights into the metabolic consequences of physical inactivity.