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Related Experiment Videos

VanG-type vancomycin-resistant Enterococcus faecalis strains isolated in Canada.

David A Boyd1, Tim Du, Romeo Hizon

  • 1National Microbiology Laboratory, Public Health Agency of Canada, Winnipeg, Manitoba.

Antimicrobial Agents and Chemotherapy
|May 26, 2006
PubMed
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Two strains of Enterococcus faecalis resistant to vancomycin were identified. One harbored a vanG operon similar to a known strain, while the other contained a novel vanG2 operon in a unique location.

Area of Science:

  • Microbiology
  • Genetics
  • Antimicrobial Resistance

Background:

  • Enterococcus faecalis is a common cause of hospital-acquired infections.
  • Vancomycin resistance in Enterococcus faecalis is a significant public health concern.
  • The vanG operon confers resistance to vancomycin.

Purpose of the Study:

  • To characterize the vancomycin resistance genes in two clinical isolates of Enterococcus faecalis.
  • To determine the genetic context and location of the vanG operon in these isolates.

Main Methods:

  • Minimum Inhibitory Concentration (MIC) testing for vancomycin.
  • Whole-genome sequencing and comparative analysis of vanG operons.
  • Plasmid and chromosomal DNA analysis.

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Main Results:

  • Enterococcus faecalis G1-0247 possessed a vanG operon 99% identical to that of E. faecalis BM4518.
  • Enterococcus faecalis N03-0233 harbored a novel vanG2 operon.
  • The vanG and vanG2 operons were located on mobile genetic elements in different chromosomal positions.

Conclusions:

  • The vanG operon is present in multiple E. faecalis strains, suggesting potential for dissemination.
  • The discovery of a novel vanG2 operon highlights the ongoing evolution of vancomycin resistance mechanisms.
  • Understanding the genetic location and context of these operons is crucial for tracking and controlling resistance spread.