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Analysis of Minerals Produced by hFOB 1.19 and Saos-2 Cells Using Transmission Electron Microscopy with Energy Dispersive X-ray Microanalysis

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Extracellular phosphate alters cementoblast gene expression.

R B Rutherford¹, B L Foster, T Bammler

¹Department of Oral Biology, School of Dentistry, University of Washington, Box 357132, Seattle, WA 98195-7132, USA. rbruth@u.washington.edu

Journal of Dental Research

|May 26, 2006

Summary

Altered extracellular phosphate levels impact cementum formation by changing gene expression in mouse cementoblasts. This study reveals key molecular pathways involved in phosphate-regulated cementogenesis.

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Isolation of Whole Cell Protein Lysates from Mouse Facial Processes and Cultured Palatal Mesenchyme Cells for Phosphoprotein Analysis

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Published on: April 1, 2022

Area of Science:

Biochemistry
Genomics
Developmental Biology

Background:

Cementum formation is crucial for tooth anchoring and is influenced by phosphate/pyrophosphate levels.
The intracellular mechanisms linking extracellular phosphate to cementum development remain unclear.

Purpose of the Study:

To investigate the temporal effects of extracellular phosphate on gene expression in mouse cementoblasts.
To identify molecular pathways affected by phosphate in cementum-forming cells.

Main Methods:

Cultured immortalized mouse cementoblasts were treated with 5 mM inorganic phosphate for 1-48 hours.
Global gene expression patterns were analyzed using DNA microarrays covering the entire mouse genome.

Main Results:

Extracellular phosphate significantly altered the expression of numerous genes.
Affected genes were enriched in Gene Ontology (GO) groups, notably transcription factor activity and Wnt signaling pathways.

Conclusions:

Extracellular phosphate concentration is a critical regulator of gene expression in cementoblasts.
Wnt signaling and transcription factor activity are key intracellular pathways modulated by phosphate during cementum formation.