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Related Experiment Videos

Myc influences global chromatin structure.

Paul S Knoepfler1, Xiao-yong Zhang, Pei Feng Cheng

  • 1Division of Basic Sciences, Fred Hutchinson Cancer Research Center, Seattle, WA 98109-4417, USA.

The EMBO Journal
|May 26, 2006
PubMed
Summary

Myc proteins are essential for maintaining active chromatin structure. Loss of Myc leads to rapid chromatin inactivation, impacting cell growth and cancer development.

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Area of Science:

  • Oncology
  • Molecular Biology
  • Epigenetics

Background:

  • Myc proto-oncogenes (c-, N-, and L-Myc) encode transcription factors regulating cell growth and proliferation.
  • Myc proteins are implicated in the development of various cancers.
  • Their function is primarily understood through binding and regulating specific target genes.

Purpose of the Study:

  • To investigate the role of Myc proteins in maintaining active chromatin.
  • To elucidate the impact of Myc disruption on chromatin structure and modifications.
  • To explore the mechanism by which Myc influences global chromatin.

Main Methods:

  • Disruption of N-myc in neuronal progenitors and other cell types.
  • Analysis of nuclear condensation and histone modifications (acetylation, methylation).

Related Experiment Videos

  • Assessment of effects of exogenous Myc, differentiation, and Myc antagonist Mad1.

    • Main Results:

    • Myc proteins are required for widespread maintenance of active chromatin.
    • N-myc disruption causes nuclear condensation and large-scale chromatin inactivation.
    • Histone hypoacetylation and altered methylation were observed upon Myc loss.
    • Effects were reversible by exogenous Myc, differentiation, or Mad1.
    • Chromatin changes occurred within 6 hours of Myc loss.
    • Myc influences chromatin partly via upregulation of histone acetyltransferase GCN5.

    Conclusions:

    • Myc proteins play a crucial role in regulating global chromatin structure.
    • This regulation provides a mechanism for Myc's broad effects on cell behavior and tumorigenesis.
    • This study presents the first evidence of oncoprotein-driven regulation of global chromatin structure.