Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Recent advances in biocatalysis by directed enzyme evolution.

Sheryl B Rubin-Pitel1, Huimin Zhao

  • 1Department of Chemical and Biomolecular Engineering, University of Illinois, Urbana, IL 61801, USA.

Combinatorial Chemistry & High Throughput Screening
|May 27, 2006
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Metabolic engineering of Rhodotorula toruloides IFO0880 improves C16 and C18 fatty alcohol production from synthetic media.

Microbial cell factories·2022
Same author

Selectively Lighting Up Singlet Oxygen via Aggregation-Induced Electrochemiluminescence Energy Transfer.

Analytical chemistry·2022
Same author

Directed Evolution of Replication-Competent Double-Stranded DNA Bacteriophage toward New Host Specificity.

ACS synthetic biology·2022
Same author

Metabolic engineering of threonine catabolism enables Saccharomyces cerevisiae to produce propionate under aerobic conditions.

Biotechnology journal·2022
Same author

Recent advances and perspectives of enzyme-based optical biosensing for organophosphorus pesticides detection.

Talanta·2021
Same author

Pathological diagnosis, differential diagnosis and origin investigation of easily misdiagnosed adult gastric duplication cysts.

Histology and histopathology·2021

Directed evolution engineers enzymes for better industrial and medical applications. This powerful technique refines enzyme activity, selectivity, and stability for specific uses, creating novel biocatalysts.

Area of Science:

  • Biochemistry
  • Biotechnology
  • Enzyme Engineering

Background:

  • Enzymes are natural biocatalysts with vast industrial and medical potential.
  • Existing enzymes often require property optimization for specific applications.
  • Directed evolution is a key technology for enzyme improvement.

Purpose of the Study:

  • To review recent advancements in directed evolution for enzyme engineering.
  • To highlight the modification of enzyme properties like activity, selectivity, and stability.
  • To focus on the creation of novel enzyme functions and products.

Main Methods:

  • Utilizing directed evolution strategies to modify enzyme characteristics.
  • Applying techniques to enhance enzyme activity and specificity (enantio- and regio-).

Related Experiment Videos

  • Improving enzyme substrate specificity, stability, and solubility.

    • Main Results:

    • Demonstrated success in altering diverse enzyme properties through directed evolution.
    • Showcased the engineering of enzymes with enhanced performance metrics.
    • Highlighted the generation of novel enzymatic activities and products.

    Conclusions:

    • Directed evolution is a versatile and powerful tool for enzyme engineering.
    • This methodology enables the tailoring of enzymes for specific industrial and medical needs.
    • Recent advances show significant progress in creating novel biocatalysts through directed evolution.