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Intergeneric relationship between the Aspergillus ochraceous virus F and the Penicillium stoloniferum virus S

Jong Wook Kim1, Eun Young Choi, Young Tae Kim

  • 1Department of Biochemistry, College of Medicine, Inha University, Incheon 402-751, South Korea. jwkim@inha.ac.kr

Virus Research
|May 27, 2006
PubMed

Insights

The Aspergillus ochraceous virus (AoV-F) fast component, previously thought to be related to Penicillium stoloniferum virus (PsV-S) via capsid proteins, is actually related through its RNA-dependent RNA polymerase (RdRp). This finding clarifies intergeneric virus relationships.

Area of Science:

  • Virology
  • Molecular Biology
  • Mycology

Background:

  • The "slow" component of Penicillium stoloniferum virus (PsV-S) was previously observed in Aspergillus ochraceous, attributed to the "fast" component of Aspergillus ochraceous virus (AoV-F).
  • This intergeneric occurrence suggested a serological relationship between AoV-F and PsV-S, assumed to be between their capsid proteins.

Purpose of the Study:

  • To investigate the molecular basis of the cross-serological relationship between AoV-F and PsV-S.
  • To characterize the genome organization and protein products of AoV dsRNA 1.

Main Methods:

  • Cloning and sequencing of AoV dsRNA 1.
  • Bioinformatic analysis including BLAST searches and phylogenetic analysis.
  • Immunoblot analysis using antiserum against PsV-S.

Main Results:

  • AoV dsRNA 1 (1754 bp) encodes a putative RNA-dependent RNA polymerase (RdRp, p63) with 81% homology to Partitivirus members.
  • Phylogenetic analysis grouped AoV-F with PsV-S.
  • Immunoblot analysis showed no cross-reactivity between AoV-F capsid protein (P3) and PsV-S antiserum.

Conclusions:

  • The previously observed cross-serological relationship between AoV-F and PsV-S is likely due to homology between their respective RNA-dependent RNA polymerases (RdRps), not their capsid proteins.
  • This study revises the understanding of intergeneric virus relationships and the mechanisms of cross-serological reactions.

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