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Related Experiment Videos

p-glycoprotein expression in malignant melanoma.

B Fuchs1, H Ostmeier, L Suter

  • 1Fachklinik Hornheide, Münster, Federal Republic of Germany.

Journal of Cancer Research and Clinical Oncology
|January 1, 1991
PubMed
Summary

Drug resistance in human melanoma is typically not due to p-glycoprotein efflux. This study found limited p-glycoprotein expression in melanoma, suggesting other mechanisms drive chemotherapy resistance.

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Area of Science:

  • Oncology
  • Molecular Biology
  • Biochemistry

Background:

  • Chemotherapy resistance in human malignancies can stem from energy-dependent efflux systems.
  • The permeability glycoprotein (p-glycoprotein), encoded by the multidrug-resistance gene MDR1, is a key component of these efflux systems.

Purpose of the Study:

  • To investigate the role of p-glycoprotein expression in primary and metastatic human melanoma.
  • To determine if p-glycoprotein expression correlates with chemotherapy response in melanoma patients.

Main Methods:

  • Utilized the monoclonal antibody C219 to assess p-glycoprotein expression in melanoma samples.
  • Correlated p-glycoprotein expression levels with patient response to chemotherapy.
  • Conducted sequential studies to monitor p-glycoprotein expression changes during and after chemotherapy.

Main Results:

  • Substantial p-glycoprotein expression was found in only 1/37 primary melanomas and 1/27 melanoma metastases.
  • Patients with negative p-glycoprotein metastases did not respond to chemotherapy.
  • One patient with significantly expressing p-glycoprotein metastases achieved complete remission of metastatic growth.
  • No significant increase in p-glycoprotein-positive cells was observed during or after chemotherapy.

Conclusions:

  • P-glycoprotein-related efflux systems are generally not the primary cause of drug resistance in human melanoma.
  • Alternative mechanisms are likely responsible for chemotherapy resistance in this malignancy.

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