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Evaluation of Intracellular Location of Reactive Oxygen Species in Solea Senegalensis Spermatozoa
Published on: March 11, 2018
Humidity resistant live-dead stain for spermatozoa
1Department of Dairy Science, University of Missouri, Columbia, 65211 USA.
Abstract:
Live-dead slides of bovine spermatozoa, prepared using conventional procedures, were found to be unstable at high relative humidities (RH) because of migration of water soluble dyes, such as eosin, into previously unstained cells At 85 percent RH, complete loss of contrast between "live" and "dead" cells occurred within 60 minutes. Comparable slides, prepared using erythrosin B and brilliant green, were found to maintain satisfactory contrast between the "live" and "dead" cells, even at 98 percent RH, throughout the seven-day humidity exposure trials.
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