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Total lipids and phospholipids in buffalo semen (Bubalus bubalis)
B C Sarmah1, M L Kaker, M N Razdan
1Department of Animal Production Physiology, Haryana Agricultural University, Hissar 125004, India.
Theriogenology
|November 1, 1983
Summary
This study quantifies lipid and phospholipid concentrations in Murrah buffalo bull semen. Phosphatidyl choline is the primary phospholipid fraction in both sperm and seminal plasma.
Area of Science:
- Reproductive Biology
- Animal Science
- Biochemistry
Background:
- Lipids play crucial roles in sperm function and fertility.
- Understanding seminal plasma composition is vital for reproductive efficiency in livestock.
Purpose of the Study:
- To quantify total lipid and phospholipid concentrations in Murrah buffalo bull spermatozoa and seminal plasma.
- To identify the major phospholipid fractions present in buffalo bull semen.
Main Methods:
- Analysis of 50 ejaculates for total lipids and 30 ejaculates for phospholipids and their fractions.
- Quantification using established biochemical assays.
Main Results:
- Sperm lipid content averaged 1.32 mg/10(9) cells, while seminal plasma averaged 1.75 mg/ml.
- Spermatozoal phospholipid content averaged 0.64 mg/10(9) cells, and seminal plasma averaged 0.69 mg/ml.
- Phosphatidyl choline was identified as the predominant phospholipid fraction in both sperm and seminal plasma.
Conclusions:
- Murrah buffalo bull semen contains significant amounts of lipids and phospholipids.
- Phosphatidyl choline is a key component of buffalo bull spermatozoa and seminal plasma, potentially influencing fertility.