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A Modified Co-Culture System for Understanding Granulosa-Theca Cell Interactions in the Bovine Ovary
Published on: September 19, 2025
Studies on follicular patterns in buffalo-heifers
G Singh1, G B Singh, S S Sharma
1Department of Obstetrics & Gynaecology College of Veterinary Science Punjab Agricultural University Ludhiana-141004 India.
Theriogenology
|November 1, 1984
Summary
Palpation per rectum revealed continuous development and atresia of large ovarian follicles in heifers. These follicular dynamics occurred regularly every 10-11 days, offering insights into bovine reproductive cycles.
Area of Science:
- Veterinary Science
- Reproductive Biology
- Animal Science
Background:
- Understanding ovarian follicular dynamics is crucial for managing cattle reproduction.
- Previous studies have focused on various aspects of follicular development, but continuous monitoring in heifers requires further investigation.
Purpose of the Study:
- To delineate the patterns of development and atresia of large ovarian follicles in pre-, peri-, and postpubertal heifers.
- To determine the frequency and timing of large follicle presence during the estrous cycle in heifers.
Main Methods:
- Rectal palpation was frequently employed to assess ovarian follicles in heifers across different pubertal stages.
- Average follicle sizes and the presence of midcycle follicles were recorded throughout the estrous cycle.
Main Results:
- Average large follicle sizes were 1.25 cm (prepubertal), 1.35 cm (peripubertal), and 1.36 cm (postpubertal).
- Midcycle follicles (>0.80 cm) were palpable in 65.39% of postpubertal heifers between days 9-13 of the estrous cycle.
- Follicle development and atresia were continuous processes, with follicles appearing every 10-11 days.
Conclusions:
- Follicular development and atresia in heifers are continuous, cyclical processes.
- Rectal palpation is an effective method for monitoring ovarian follicular dynamics in heifers.
- These findings contribute to a better understanding of bovine reproductive physiology.

