Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Chemically defined and serum supplemented media effects on mouse embryo development.

R D Bates1, J A Kontio, W R Dukelow

  • 1Endocrine Research Center Michigan State University East Lansing, MI 48824 USA.

Theriogenology
|April 1, 1985
PubMed
Summary

The study compared mouse embryo growth in different media. TALP medium significantly improved early embryo development, and human cord serum (HCS) supported more cleavage divisions than fetal calf serum (FCS).

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Accumulation of 2-deoxy-D-glucose by primate oocytes fertilized in vitro.

American journal of primatology·2020
Same author

Brief report: Synthesis of ribonucleic acid in oocytes collected from squirrel monkeys and humans following chorionic gonadotropin administration.

American journal of primatology·2020
Same author

Immunological response and ovarian histology of squirrel monkeys (Saimiri sciureus) immunized with porcine zona pellucida ZP3 (M<sub>r</sub> = 55,000) macromolecules.

American journal of primatology·2020
Same author

In vitro fertilization analysis of squirrel monkey oocytes produced by various follicular induction regimens and the incidence of triploidy.

American journal of primatology·2020
Same author

Reflections on a century of primatology.

American journal of primatology·1999
Same author

Incorporation of a GnRH agonist, leuprolide acetate, into regimens with exogenous gonadotropins to produce ovarian stimulation and ovulation in the nonpregnant squirrel monkey.

American journal of primatology·1999

Area of Science:

  • Developmental Biology
  • Reproductive Science
  • In Vitro Culture

Background:

  • Optimizing in vitro culture media is crucial for early embryo development.
  • Serum supplementation in media can influence embryo viability and progression.
  • Comparing defined media with serum-supplemented media is essential for improving culture conditions.

Purpose of the Study:

  • To evaluate the efficacy of three different media (TC199, CMRL-1066, and TALP) for mouse embryo culture.
  • To compare the effects of human cord serum (HCS) and fetal calf serum (FCS) on early mouse embryo development.
  • To determine if a defined medium (TALP) can support or enhance embryo growth compared to serum-supplemented media.

Main Methods:

  • Mouse embryos at two, four, and eight-cell stages were cultured in TC199, CMRL-1066, and TALP media.

Related Experiment Videos

  • The influence of 20% human cord serum (HCS) and fetal calf serum (FCS) was assessed in TC199 and CMRL-1066.
  • Embryo cleavage progression was monitored over 24 hours to assess developmental rates.

    • Main Results:

    • No significant difference in growth rates was observed between media for four and eight-cell embryos.
    • TALP medium significantly increased the proportion of two-cell embryos undergoing cleavage compared to other media.
    • Human cord serum (HCS) significantly promoted more cleavage divisions than fetal calf serum (FCS).

    Conclusions:

    • The defined medium TALP supports equal or improved growth rates for early mouse embryos in vitro.
    • TALP medium demonstrates potential for enhanced early embryo development compared to serum-supplemented media.
    • Human cord serum is a more effective supplement than fetal calf serum for promoting early embryo cleavage divisions.