Normal development of fetuses resulting from Holstein semen processed for sex separation

Semen from two high fertility Holstein bulls with extensive histories in artificial insemination was specially processed for the purpose of sexing and then frozen. The semen was used to inseminate 200 open crossbred beef heifers carefully selected for reproductive soundness from a much larger group. Animals were inseminated alternately with the semen from one of the two bulls and subsequently slaughtered. There were 96 fetuses, ranging in age from 63 to 88 days, which were carefully examined for general appearance, fetal crown-rump length and body weight. There was no effect of sire on fetal size, but the difference between fetal sexes was significant, with the male fetus being appreciably heavier. The regression of fetal length on fetal age was linear and the regression of fetal weight on fetal age was curvilinear. Fetal age accounted for 92% to 96% of the variation in fetal weight and also in crown-rump length.