Comparative studies on the superovulatory effect of PMSG and FSH in water buffalo (Bubalus bubalis )

A total of thirty-eight lactating water buffalo cows were treated in four experiments simultaneously either with FSH (first group) or PMSG(second group). To the first group (half of the animals), a total dose of 40 mg FSH-P at 12-hr intervals was given i.m. within a 4-day period. The second group was treated i.m. with 3000 IU PMSG (Gestyl). Forty-eight hours after initiation of the superovulatory treatment all buffaloes were given 500 ug Cloprostenol. Fi seen buffaloes from the FSH-treated group (78.9%) and 17 from the second group (89.5%) came into heat at average PGF 2 alpha/standing heat intervals of 42.8+/-1.48 and 44.8+/-2.31, respectively. Superovulatory treatment resulted in meath number of 4.3+/-0.87 and 1.9+/-0.50 CL and 0.5+/-0.24 and 2.2+/-0.82 follicles for the first and second group. Twenty-five eggs were recovered after non-surgical flushing from 8 of 13 flushes in the first group and all except one were fertilized and classified as good embryos. Twelve eggs were recovered from 4 of 11 flushes in the second group and 11 of the eggs were fertilized and 10 of them classified as good ones.