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Related Experiment Videos

Lamb production using superovulation, embryo bisection, and transfer.

P Chesné1, G Colas, Y Cognié

  • 1I.N.R.A., Station de Physiologie Animale, 78350 Jouy-en-Josas, France.

Theriogenology
|May 1, 1987
PubMed
Summary

This study bisected sheep embryos to create identical twin lambs, achieving a 72% lambing rate and an overall yield of 118%. Embryo collection day influenced the success rate, with later collection yielding more lambs.

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Area of Science:

  • Reproductive Biology
  • Embryology
  • Animal Science

Background:

  • Superovulation protocols are crucial for increasing the number of embryos available for research.
  • Monozygotic twinning can be induced by embryo manipulation, offering genetically identical subjects.

Purpose of the Study:

  • To evaluate the efficiency of bisecting sheep embryos to produce monozygotic twin lambs.
  • To determine the optimal embryonic stage for embryo bisection and transfer.

Main Methods:

  • Donor ewes were superovulated using follicle stimulating hormone-pituitary (FSH-P).
  • Embryos (Days 8, 9, or 10) were bisected to create monozygotic twin pairs.
  • Embryo pairs were surgically transferred into recipient ewes synchronized to the same physiological stage.

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Main Results:

  • A 72% lambing rate was achieved from 39 recipient ewes.
  • 64% of lambings resulted in twin pairs (18 pairs), while 36% produced single lambs.
  • Overall yield was 118%, with higher yields observed for later-stage embryos (Day 10: 131%).

Conclusions:

  • Embryo bisection is an effective method for producing monozygotic twin lambs in sheep.
  • Embryonic age at the time of bisection impacts the success rate and overall yield.
  • This technique offers a valuable tool for experimental animal production, ensuring genetic uniformity.