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Updated: Aug 8, 2026
In Vitro Culture Strategy for Oocytes from Early Antral Follicle in Cattle
Published on: July 8, 2020
Recovery and culture of ova from Brucella abortus infected cows
D A Stringfellow1, V S Panangala, P A Galik
1Department of Microbiology, College of Veterinary Medicine, Auburn University, AL 36849, USA; Department of Microbiology, College of Veterinary Medicine, Auburn University, AL 36849, USA.
Abstract:
One hundred and ninety-two ova were collected from 12 mixed-breed beef and dairy cows that had been artificially infected with Brucella abortus Biotype 1 Strain 2308. Each donor cow was treated for superovulation, and nonsurgical collections were performed on one, two or three occasions. A total of 27 nonsurgical collections on Days 6 through 12 (estrus = Day 0) resulted in the recovery of an average of 7.1 ova (4.4 fertilized and 2.7 nonfertilized). Ova were washed ten times in groups of not more than 10 and cultured for the isolation of B. abortus. A portion of the recovery medium, including sedimented uterine debris from each collection, was also cultured for isolation of the organism. Brucella abortus was not detected in any sample of the recovery medium or in any group of ova.
