Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Carbohydrate synthesis inhibitors decrease interleukin 1-stimulated lymphocyte binding to endothelial cells.

R Renkonen1, J Ustinov

  • 1Department of Bacteriology, University of Helsinki, Finland.

European Journal of Immunology
|March 1, 1991
PubMed
Summary

Carbohydrates on endothelial cells are crucial for lymphocyte homing. Inhibiting specific N-linked carbohydrate processing significantly reduces lymphocyte binding, highlighting their role in immune cell trafficking.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Ethinylestradiol in combined hormonal contraceptive has a broader effect on serum proteome compared with estradiol valerate: a randomized controlled trial.

Human reproduction (Oxford, England)·2022
Same author

Thromboinflammatory changes in plasma proteome of pregnant women with PCOS detected by quantitative label-free proteomics.

Scientific reports·2019
Same author

Birth decade affects the sensitization pattern and asthma risk in Finnish adult population.

Allergy·2017
Same author

Low lymphatic vessel density associates with chronic rhinosinusitis with nasal polyps.

Rhinology·2017
Same author

Sensitization pattern affects the asthma risk in Finnish adult population.

Allergy·2015
Same author

Gremlin-1 associates with fibrillin microfibrils in vivo and regulates mesothelioma cell survival through transcription factor slug.

Oncogenesis·2013

Area of Science:

  • Immunology
  • Glycobiology
  • Cell Biology

Background:

  • Lymphocyte extravasation (homing) is a critical process in immune surveillance.
  • Endothelial cell surface proteins involved in lymphocyte binding are heavily glycosylated.
  • The role of specific carbohydrate structures in lymphocyte-endothelial cell interactions remains to be fully elucidated.

Purpose of the Study:

  • To investigate the role of N-linked carbohydrate motifs on endothelial cells in mediating lymphocyte binding.
  • To determine the impact of inhibiting specific carbohydrate processing pathways on lymphocyte homing.

Main Methods:

  • Endothelial cells were treated with N-glycosidase F to remove N-linked carbohydrates.
  • Specific inhibitors of carbohydrate processing, including castanospermine (CAST), 1-deoxymannojirimycin (DMN), and swainsonine (SW), were used to generate distinct N-linked carbohydrate structures.

Related Experiment Videos

  • Lymphocyte binding to treated endothelial cells was quantified.

    • Main Results:

    • Removal of all N-linked carbohydrates significantly reduced interleukin 1-induced lymphocyte binding.
    • CAST treatment, generating glucose-capped high-mannose oligosaccharides, decreased lymphocyte binding by 85%.
    • DMN treatment, leading to high-mannose oligosaccharides without terminal glucose, similarly inhibited lymphocyte binding.
    • SW treatment, resulting in hybrid-type glycoproteins, showed only a slight decrease in lymphocyte binding.

    Conclusions:

    • N-linked carbohydrates on endothelial cells play a significant role in lymphocyte binding.
    • Specific carbohydrate structures, particularly those with terminal glucose residues, are important for efficient lymphocyte homing.
    • Targeting these carbohydrate motifs could offer novel strategies for modulating immune cell trafficking.