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24-Hour cooled storage of equine embryos.
P L Sertich1, L B Love, M R Hodgson
1Hofmann Center for Reproductive Studies, University of Pennsylvania - New Bolton Center, Kennett Square, PA 19348, USA.
Theriogenology
|January 1, 1988
Summary
Storing equine embryos for 24 hours at 4-6°C before transfer resulted in live foals. This equine embryo preservation method shows promise for reproductive technologies.
Area of Science:
- Reproductive Biology
- Embryology
- Veterinary Science
Background:
- Efficient equine embryo cryopreservation is crucial for reproductive technologies.
- Short-term storage methods are needed to facilitate equine embryo transfer programs.
Purpose of the Study:
- To evaluate the viability of equine embryos after short-term hypothermic storage.
- To assess the pregnancy and foaling rates following the transfer of stored equine embryos.
Main Methods:
- Equine embryos were collected 7 days post-ovulation via transcervical uterine flush.
- Embryos were stored for 24 hours at 4-6°C in a specialized transport container.
- Stored embryos were transcervically transferred into recipient mares; control group received fresh embryos.
Main Results:
- Three live, normal foals were born from 16 stored embryos transferred.
- Ultrasonography detected six pregnancies at 14 days from stored embryos.
- Five foals were born from 16 control embryos transferred without storage.
Conclusions:
- Short-term hypothermic storage (24 hours at 4-6°C) is a viable method for equine embryos.
- This preservation technique can lead to successful pregnancies and live foals.
- Further research can optimize equine embryo handling and transport protocols.