Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Exposure of bovine embryos to trypsin during washing does not decrease embryonic survival.

S E Echternkamp1, S M Kappes, R R Maurer

  • 1USDA, ARS, NPA, Roman L. Hruska U.S. Meat Animal Research Center Clay Center, NE 68933 USA.

Theriogenology
|July 1, 1989
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Possible role of IGF2 receptors in regulating selection of 2 dominant follicles in cattle selected for twin ovulations and births.

Domestic animal endocrinology·2013
Same author

Effect of postweaning diet on ovarian development and fertility in replacement beef heifers.

Journal of animal science·2013
Same author

Identification of an ionotropic glutamate receptor AMPA1/GRIA1 polymorphism in crossbred beef cows differing in fertility.

Journal of animal science·2013
Same author

MicroRNA expression profile in bovine cumulus-oocyte complexes: possible role of let-7 and miR-106a in the development of bovine oocytes.

Animal reproduction science·2012
Same author

Increased abundance of aromatase and follicle stimulating hormone receptor mRNA and decreased insulin-like growth factor-2 receptor mRNA in small ovarian follicles of cattle selected for twin births.

Journal of animal science·2012
Same author

Factors affecting pregnancy rate to estrous synchronization and fixed-time artificial insemination in beef cattle.

Journal of animal science·2011
Same journal

Environmentally relevant polycyclic aromatic hydrocarbon mixtures disrupt ovarian endocrine function in the domestic hen (Gallus gallus domesticus).

Theriogenology·2026
Same journal

Sirtuin 3 alleviates ovarian granulosa cell senescence through regulating mitochondrial quality control.

Theriogenology·2026
Same journal

Heat stress on oocyte collection day has a greater impact on the conception rate than on embryo transfer day in bovine OPU-IVF programs.

Theriogenology·2026
Same journal

Redox potential in animal andrology: An experimental study on boar semen.

Theriogenology·2026
Same journal

Divergent molecular strategies underlie bovine blastocyst hatching and cryopreservation stress adaptation revealed by integrated multi-omics.

Theriogenology·2026
Same journal

Acute thermal stress alters sperm swimming activity, viability, and gene expression in blue catfish, Ictalurus furcatus.

Theriogenology·2026
See all related articles

Bovine embryo washing with trypsin does not harm development. Trypsin treatment before cryopreservation significantly improved pregnancy rates in frozen-thawed bovine embryos. This suggests a potential benefit for embryo cryopreservation.

Area of Science:

  • Veterinary Reproduction
  • Embryology
  • Cryobiology

Background:

  • Bovine embryo washing is a critical step before transfer or cryopreservation.
  • The use of proteolytic enzymes like trypsin during washing requires careful evaluation for potential adverse effects on embryo viability.

Purpose of the Study:

  • To determine if exposure to trypsin during zona pellucida-intact bovine embryo washing affects subsequent survival and development.
  • To assess the impact of trypsin washing on fresh embryo transfer and cryopreservation outcomes.

Main Methods:

  • Bovine embryos (Grade 1 & 2) were collected and washed using two protocols: control (BSA-PBS) and experimental (trypsin in Hank's solution followed by BSA-PBS).
  • Embryos were either transferred fresh to recipients or cryopreserved and transferred later.

Related Experiment Videos

  • Cryopreserved embryos were also cultured in vitro for 72 hours to evaluate development.

    • Main Results:

    • No significant difference in pregnancy rates between fresh control-washed and trypsin-washed embryos (51.0% vs 56.3%).
    • Significantly higher pregnancy rates (P<0.05) were observed for trypsin-treated embryos before cryopreservation compared to control-washed embryos (68.2% vs 38.5%).
    • In vitro survival and development of cryopreserved embryos did not differ between the two washing groups.

    Conclusions:

    • Short-term exposure (2-3 minutes) to trypsin during bovine embryo washing does not detrimentally affect embryonic development.
    • Trypsin treatment prior to cryopreservation may enhance the success rate of frozen-thawed bovine embryos.
    • The findings support the potential utility of trypsin washing in bovine embryo cryopreservation protocols.