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Related Experiment Videos

Urinary eCG patterns in the mare during pregnancy.

J F Roser1, R M Lofstedt

  • 1Department of Animal Science University of California Davis, CA 95616 USA.

Theriogenology
|October 1, 1989
PubMed
Summary
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Equine chorionic gonadotropin (eCG) in mare urine mirrors plasma levels during early pregnancy. This finding supports urine analysis for monitoring equine pregnancy using assays like ELISA and RIA.

Area of Science:

  • Reproductive Biology
  • Endocrinology
  • Animal Science

Background:

  • Equine chorionic gonadotropin (eCG) is a key hormone in equine pregnancy.
  • Accurate detection methods are crucial for monitoring pregnancy in mares.
  • Previous studies focused primarily on plasma eCG levels.

Purpose of the Study:

  • To analyze equine chorionic gonadotropin (eCG) levels in both plasma and urine of pregnant mares.
  • To compare the efficacy of radioimmunoassay (RIA) and enzyme-linked immunospecific assay (ELISA) for detecting urinary eCG.
  • To establish the correlation between urinary and plasma eCG profiles during gestation.

Main Methods:

  • Blood and urine samples were collected from pregnant and cyclic mares at frequent intervals.
  • Radioimmunoassays (RIA) were developed for plasma and urine eCG detection.

Related Experiment Videos

  • A commercial dipstick enzyme-linked immunospecific assay (ELISA) was adapted for urine eCG analysis.

    • Main Results:

    • Urinary and plasma eCG concentrations in pregnant mares increased significantly around Day 40, peaked by Day 60, and declined by Day 200.
    • Both RIA and ELISA detected similar eCG profiles in urine, correlating with plasma levels.
    • The dipstick ELISA showed higher reliability for plasma than urine, but both tested positive during peak eCG days.

    Conclusions:

    • Urinary eCG concentrations closely follow plasma eCG profiles in mares during the first trimester of pregnancy.
    • Urine analysis using RIA and ELISA can be a viable, non-invasive method for monitoring equine pregnancy.
    • Further validation of the dipstick ELISA for equine urine eCG is warranted.