A simplified laparoscopy technique for repeated ovarian observation in the water buffalo (Bubalus bubalis)

A simplified technique of laparoscopy was developed for ovarian observation in the riverine buffalo, through a right paralumbar incision. The technique differed from previously described ones in that it involved only a single puncture and required no abdominal insufflation. A Hopkins 0 degrees forward viewing endoscope (5.5 mm x 500 mm) in combination with an endoscope sheath having a built-in instrument channel, and a long flexible forceps (630 mm) were used. Of the 23 observation attempts on 13 buffalo, 21 successful observations were conducted. Laparoscopies were performed using a combination of Xylazine, local infiltration and epidural anesthesia in a standing position. Six repeated observations were made within a 21-day period on 1 buffalo, with no postoperative complications. Observation of both left and right ovaries was possible through the same puncture. The technique was useful in buffalo to confirm ovarian structures which could not be determined with certainty through palpation per rectum. Our results suggest that the single puncture laparoscopy technique can be safely used for repeated ovarian examination in the water buffalo.